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Antibodies sure to the blocking peptide not bind to your epitope over the focus on protein. By evaluating the staining from your blocked antibody versus the antibody on your own, you can see which staining is certain.
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This manual outlines even further achievable optimization of mobile and immunoassay parameters to make sure the absolute best results are...
This information outlines further more achievable optimization of mobile and immunoassay parameters to be certain the best possible benefits are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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Blocking peptides are peptides that bind specially towards the target antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress via S section.
Overexpression of this gene has been noticed in many tumors, which leads to chromosome instability, and therefore may lead to tumorigenesis. This protein was found to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in promoting cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two added splice variants were documented but in-depth nucleotide sequence data isn't still available.
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